Carbohydrate loading diet is a diet that is to increase the amount of fuel stored in muscles to improve athletic performance in events. It was the first diet that was popular in the late 60s. During this study by Bosch, they compared liver glucose turnover, blood glucose oxidation, and muscle glycogen utilization. His goal was to calculate the effects of pre-exercise Carbohydrate Loading on the things that were listed above, during prolonged exercise and to see how it might build up performance during a continuance of exercise. The test had around fifteen subjects, of which were males that were cyclists. Each cyclist was randomly assigned to go to two different groups, the Carbohydrate loading diet group, and the non-carbohydrate loading diet group. In one of the tables gave out the pieces of information of the groups, such as the ages, average of body fat, VO2 max and many more. After getting the information the subjects would get tested by doing a 180 min cycling and get the data that was needed to compare the two groups. Every 22 mins the researchers will put in the data of both groups and see if they increase in numbers or decrease. By looking at the graphs and data, they conclude that the Carbohydrate loading does not influence the blood glucose, it did decrease it from 5 to 4.5 however for the non-carbohydrate loading it decreases from 5 to 3, that was a huge drop. For the muscle glycogen, it was higher in the loading than the non-loading throughout the whole 180 min exercise. However, there were some subjects that were unable to complete the exercise and became exhausted by the time they reached 120 minutes, and they were from the non-carbohydrate loading group and there were four subjects that could not complete the exercise.For the liver glucose turnover, they conclude that there was no effect, however, it exceeded glucose Rox during only the first 75 mins of the exercise.