In this lab report “The Examination of plant seed germination” the Ph and rate of gemmation are related to the growth of a seed is affected by many different things and one of them being the pH level. Just like mammals or any other living organism when it comes to things like temperature, space and light vs dark. Seeds have a tolerance when it comes to pH levels. Every seed is different, some could probably flourish in a high pH levels and some probably flourish in the lower pH levels; it just depends on the certain seed. The point of this lab is to determine on what level of The Ph and rate of are gemmation are related; higher concentration of law Ph solute, merger, in aqueous solution will result in a low rate of successful gemmating.We used six sterile petri dishes with filter paper as the germination substrate for all radish seeds.
Three of the petri dishes were saturated in vinegar and the other three dishes were saturated in tap water. Each petri dish contained ten seeds and was watered with 10 ml of solution twice a day. The radish seeds were allowed five days for germination and seedling growth. The samples with the vinegar were place in the lab draw which caused darkness. The samples with the tap water was also placed in a dark area also. Following the five-day treatments, we calculated only a small percent of germination and measured the growth of any seedlings. The seedling growth was measured from the base of the coumarin to the top of the stem, excluding leaf length.
A comparison of the various means was used to identify differences between both treatments. The following tables include radish growth per day for seven days in the three different PH/growth mediums. As shown the 100% water medium with a PH of 7 was the only medium to produce radish seed growth.The results obtained from our experiment prove to be in support of our hypothesis.
As the acidity of our solution increased, the germination of our beet seeds was inhibited. The control group germinated abundantly, as expected, since we used distilled water as our solution. The pH for distilled water is 7.
0 as opposed to the pH of vinegar which is approximately 2.4. Seeds require 3 things for the process of germination: water, the appropriate temperature, and the appropriate environment. (Seed Germination, 2013) According to a similar experiment published in the Oxford Academic Journal of General Botany, seed germination and root elongation were both inhibited in solutions of pH less than or equal to 4.
0 after about 5 days but increased almost linearly with time in solutions with a pH greater than 5.0 (Oxford, 2012). Our results matched the results from this Oxford study of acidity. Although our hypothesis tested correctly, there are some factors that could have potentially changed the outcome. For example, if we had diluted our vinegar solution even further we may have seen more germination results.
With respect to potential experimental error, our results remained consistent with similar experiments done previously which further strengthens our hypothesis. The results of our experiment now raise the question: could seed germination be possible in a solution with a pH higher than 7.1?
