MICROSCOPY;  Microscopy
is a techniques that help to view microorganism clearly whichcant see by naked
eye, using microbes to view objects. Microscope is an optical instrument that uses a
lens or a combination of lenses to produce magnified images of small objects,
especially of objects too small to be seen by the unaided eye. There is a many
branches of microscopy but the one of the compound microscope is commonly used

technique which is frequently used for the separation of particles from the
solution according to their size, shape, density, viscosity of the medium and
rotor speed .Different speeds were used for different samples.The particles are
suspended in a liquid medium and placed in a centrifuge tube. The
tube is then placed in a rotor and spun at a define speed.Separation
through sedimentation could be done naturally with the earth gravity,
nevertheless, it would take ages. Centrifugation is making that natural
process much faster. Rotation of the rotor about a central axis creates a centrifugal
force upon the particles in the suspension

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CHROMOTGRAPHY;It is a technique
to tell of the group of analytical separation techniques used to detect or
identify volatile substances in the gas phase. In gas chromatography, the constituent
of a sample are dissolved in a solvent and vaporized in order to separate the
analytes by distributing the sample between two phases: a stationary phase and
a mobile phase.

The mobile
phase is a chemically inert gas that serves to take the molecules of the
analyte through the heated column. Gas chromatography is one of the sole forms
of chromatography that does not utilize the mobile phase for interacting with
the analyte.

stationary phase is either a solid adsorbant, termed gas-solid chromatography (GSC),
or a liquid on an inert support, termed gas-liquid chromatography (GLC).

FLAME PHOTOMETRY;A branch of atomic spectroscopy ,
the oldest instrumental method for the identification of elements. This techniques
was introduced in the mid of 19th Century during which Bunsen and Kirchhoff
showed that the radiation emitted from the flames depends on the characteristic
element present in the flame. In this technique the samples in solution are excited to develop a line emission spectra by introduction into a flame. A
photoelectric flame photometer is a device used for inorganic chemical
analysis to determine the concentration of certain metal ions, example sodium,
potassium, lithium, and calcium. Group 1 and Group 2 metals are sensitive to Flame
Photometry due to their low excitation energies


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