Preeclampsia in 3–5% of pregnant women worldwide, is

Preeclampsia (PE), as
multisystem pathological condition that occurs in 3–5% of pregnant women worldwide,
is clinically diagnosed by maternal hypertension and proteinuria 1, 2. Preeclampsia may progress to
eclampsia (convulsive form) due to late presenta­tion, delayed diagnosis, and
delayed treatment; therefore, there is a need to identify reliable hall­marks
for the early diagnosis of preeclampsia and to develop efficient treatment

The precise
pathogenic mechanisms underlying preeclampsia is still undetermined. However,
immune maladaptation, inadequate placental development and trophoblast
invasion, placental ischemia, oxidative stress, and thrombosis are all thought
to represent key factors in the development of the
disease. Furthermore, these components have genetic factors that may be involved
in these pathogenic changes4.

We Will Write a Custom Essay Specifically
For You For Only $13.90/page!

order now

and apoptosis (programmed cell death) are absolutely necessary components of
the trophoblast life cycle. There are aberrant cell turnover including an
increased apoptosis in placental trophoblast of preeclamptic pregnancies5.

differentiation of cytotrophoblast cells during their invasion of the
spiral uterine
arteries results in a decreased placental size and restricted
utero-placental blood flow, which does not meet the needs of the growing fetus.
As a consequence, hypoxia of the placenta is
followed by an increase in syncytiotrophoblast apoptosis and necrosis6. 

The precise
intracellular mechanisms that promote apoptosis in PE are unknown7. TP53, as a
tumor-suppressor gene, is a key component in cell cycle progression and the
induction of apoptosis8. P53 protein
is an important transcription factor that regulates growth arrest, apoptosis
and DNA repair under cell stress conditions, and then it is phosphorylated and
acetylated at multiple sites to activate downstream target genes9. It was shown
that the level of p53 was increased in placentas during complicated pregnancies;
this finding highlighted the role of P53 in trophoblast apoptosis10. The increased
levels of p53, promotes the downstream transcription of elements involved in
apoptosis and cell-cycle arrest, including p21, a cyclin-dependent kinase


The TP53 gene
is located on chromosome 17 and encodes a 53 kDa protein containing 393 amino
acids. The TP53 gene has various single nucleotide polymorphisms (SNPs) with
probable functional effects.  A
well-recognized polymorphism in TP53 gene (P72R, rs1042522) is characterized by
a G>C substitution at codon 72 and is located in the transactivation domain
of the p53 protein that could affect activity of this protein11.

The most
commonly studied polymorphism of p21 gene, rs1801270 (C98A), is a C>A
substitution at codon 31 (serine to arginine replacement) of P21 protein. This
substitution affects the DNA binding zinc finger motif and may alter expression
and activity of P21. Another P21 polymorphism rs1059234 (C70T) located 20
nucleotides downstream of the stop codon within the 3’untranslated region. It
is considered that, this region is an important site for cell differentiation,
proliferation, and tumor suppression12.


Although several
studies have suggested the importance of the apoptosis pathways and its related
genes in the regulation of PE13, 14; there is no
studies evaluating polymorphisms in P21 gene and correlation with PE
development. In addition, the published reports on the association between P53
gene polymorphisms and PE are sparse in number15. In this
study, we analyzed the association of TP53 (rs1042522) and P21 (rs1801270 and rs1059234)
genes polymorphisms and risk of PE development.